Oligonucleotide microarray analysis of differential transporter regulation in the regenerating rat liver
Identifieur interne : 000A86 ( Istex/Checkpoint ); précédent : 000A85; suivant : 000A87Oligonucleotide microarray analysis of differential transporter regulation in the regenerating rat liver
Auteurs : Olaf Dransfeld [Allemagne] ; Thor Gehrmann [Allemagne] ; Karl Köhrer [Allemagne] ; Gerald Kircheis [Allemagne] ; Claudia Holneicher [Allemagne] ; Dieter H Ussinger [Allemagne] ; Matthias Wettstein [Allemagne]Source :
- Liver International [ 1478-3223 ] ; 2005-12.
English descriptors
- Teeft :
- Acid, Acid transporter, Amino, Amino acid transport system, Amino acid transporter system, Amino acid transporters, Amino acid uptake, Amino acids, Anion, Asct2, Betaine, Bgt1, Bile, Bile acid uptake, Bile salt export, Biliary, Bsep, Cathepsin, Cation, Cdna, Cell proliferation, Cell volume increase, Cellular hydration state, Connexin, Control livers, Cotransporter, Cycle number, Cyclin, Different time points, Differential regulation, Downregulation, Dransfeld, Excretion, Gene, Gene expression, Gene regulation, Glucose, Glucose transporter, Growth factor binding protein, Hepatectomy, Hepatic, Hepatic transport systems, Hepatocyte, Hepatocyte proliferation, Hepatology, Hybridization, Junction membrane channel protein, Junction protein, Kinase, Liver, Liver homogenates, Liver parenchymal cells, Liver regeneration, Microarray, Microarray analysis, Microarray data, Monoclonal antibody, Mrna, Mrna expression, Mrp6, Multidrug, Multidrug resistance protein, Name symbol gene, Nkcc1, Nkcc1 protein, Ntcp, Nuclear receptors, Oatp, Oatp5, Oligonucleotide, Oligonucleotide microarray analysis, Organic anion, Organic anion transporter, Organic cation transporter, Osmolyte, Osmolyte transporters, Parenchymal, Partial hepatectomy, Perfused, Physiol, Polypeptide, Protein kinase, Receptor, Regeneration, Ribosomal protein, Sham, Sham operation, Smit, Solute carrier family, Statistical analysis, Strong induction, Tissue inhibitor, Transport activities, Transporter, Transporter regulation, Uorescence, Uorescence signal, Upregulation, Uptake, Wettstein.
Abstract
Abstract: Aims: The aim of this study was to investigate the regulation of hepatic transport systems during liver regeneration. Methods: A DNA oligonucleotide microarray was developed with probes for 400 transcripts. Data were confirmed using real‐time PCR and on a functional level in the perfused rat liver. Liver homogenates were taken 3–48 h following 2/3‐hepatectomy in rats and compared with sham‐operated and non‐operated controls. Results: A more than two‐fold increase or decrease of expression was obtained in 183 genes following partial hepatectomy and in 16 genes in sham‐operated rats. A strong induction during liver regeneration was detected for the amino acid transporters LAT4, SN2 and sodium‐dependent neutral amino acid transporter (ASCT)2, whereas amino acid transport system (ATA)2 and ATA3 expressions remained unchanged. The upregulation of ASCT2 may be responsible for the increase in sodium‐dependent neutral amino acid influx important for liver cell proliferation. Expression of the osmolyte transporters Smit, TauT and Bgt1 was almost unchanged indicating that osmolytes are not involved in the cell volume increase during liver regeneration. The basolateral bile salt transporter Ntcp messenger RNA (mRNA) was significantly downregulated, whereas bile salt export pump (Bsep) and multidrug resistance protein (Mrp)2 expressions remained almost unchanged. An increased mRNA expression following partial hepatectomy was detected for organic anion transporting polypeptide (Oatp)5, Octn1, Octn2 and SGLT2. In contrast, Mrp6, Oatp 2, Oatp 3, Oatp 4 and Oatp 7 were downregulated. A five‐fold upregulation at the protein level was shown for the Na+–K+–2Cl− cotransporter sodium‐potassium‐2‐chloride cotransporter (NKCC1). Conclusions: The data show a differential regulation of hepatic transport systems during liver regeneration.
Url:
DOI: 10.1111/j.1478-3231.2005.01158.x
Affiliations:
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Dieter" uniqKey="H Ussinger D" first="Dieter" last="H Ussinger">Dieter H Ussinger</name></author><author><name sortKey="Wettstein, Matthias" sort="Wettstein, Matthias" uniqKey="Wettstein M" first="Matthias" last="Wettstein">Matthias Wettstein</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:5C6B0BB7BD9B2690780ABF0A335855985656B8DB</idno><date when="2005" year="2005">2005</date><idno type="doi">10.1111/j.1478-3231.2005.01158.x</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-PF9QFF2J-H/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">002350</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002350</idno><idno type="wicri:Area/Istex/Curation">002350</idno><idno type="wicri:Area/Istex/Checkpoint">000A86</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000A86</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" 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Duesseldorf</wicri:regionArea><wicri:noRegion>Duesseldorf</wicri:noRegion><wicri:noRegion>Duesseldorf</wicri:noRegion></affiliation></author><author><name sortKey="Holneicher, Claudia" sort="Holneicher, Claudia" uniqKey="Holneicher C" first="Claudia" last="Holneicher">Claudia Holneicher</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Clinic for Gastroenterology, Hepatology and Infectiology, University Hospital Duesseldorf, Duesseldorf</wicri:regionArea><wicri:noRegion>Duesseldorf</wicri:noRegion><wicri:noRegion>Duesseldorf</wicri:noRegion></affiliation></author><author><name sortKey="H Ussinger, Dieter" sort="H Ussinger, Dieter" uniqKey="H Ussinger D" first="Dieter" last="H Ussinger">Dieter H Ussinger</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Clinic for Gastroenterology, Hepatology and Infectiology, University Hospital Duesseldorf, Duesseldorf</wicri:regionArea><wicri:noRegion>Duesseldorf</wicri:noRegion><wicri:noRegion>Duesseldorf</wicri:noRegion></affiliation></author><author><name sortKey="Wettstein, Matthias" sort="Wettstein, Matthias" uniqKey="Wettstein M" first="Matthias" last="Wettstein">Matthias Wettstein</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Clinic for Gastroenterology, Hepatology and Infectiology, University Hospital Duesseldorf, Duesseldorf</wicri:regionArea><wicri:noRegion>Duesseldorf</wicri:noRegion><wicri:noRegion>Duesseldorf</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Liver International</title><title level="j" type="alt">LIVER INTERNATIONAL</title><idno type="ISSN">1478-3223</idno><idno type="eISSN">1478-3231</idno><imprint><biblScope unit="vol">25</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="1243">1243</biblScope><biblScope unit="page" to="1258">1258</biblScope><biblScope unit="page-count">16</biblScope><publisher>Munksgaard International Publishers</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2005-12">2005-12</date></imprint><idno type="ISSN">1478-3223</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1478-3223</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acid</term><term>Acid transporter</term><term>Amino</term><term>Amino acid transport system</term><term>Amino acid transporter system</term><term>Amino acid transporters</term><term>Amino acid uptake</term><term>Amino acids</term><term>Anion</term><term>Asct2</term><term>Betaine</term><term>Bgt1</term><term>Bile</term><term>Bile acid uptake</term><term>Bile salt export</term><term>Biliary</term><term>Bsep</term><term>Cathepsin</term><term>Cation</term><term>Cdna</term><term>Cell proliferation</term><term>Cell volume increase</term><term>Cellular hydration state</term><term>Connexin</term><term>Control livers</term><term>Cotransporter</term><term>Cycle number</term><term>Cyclin</term><term>Different time points</term><term>Differential regulation</term><term>Downregulation</term><term>Dransfeld</term><term>Excretion</term><term>Gene</term><term>Gene expression</term><term>Gene regulation</term><term>Glucose</term><term>Glucose transporter</term><term>Growth factor binding protein</term><term>Hepatectomy</term><term>Hepatic</term><term>Hepatic transport systems</term><term>Hepatocyte</term><term>Hepatocyte proliferation</term><term>Hepatology</term><term>Hybridization</term><term>Junction membrane channel protein</term><term>Junction protein</term><term>Kinase</term><term>Liver</term><term>Liver homogenates</term><term>Liver parenchymal cells</term><term>Liver regeneration</term><term>Microarray</term><term>Microarray analysis</term><term>Microarray data</term><term>Monoclonal antibody</term><term>Mrna</term><term>Mrna expression</term><term>Mrp6</term><term>Multidrug</term><term>Multidrug resistance protein</term><term>Name symbol gene</term><term>Nkcc1</term><term>Nkcc1 protein</term><term>Ntcp</term><term>Nuclear receptors</term><term>Oatp</term><term>Oatp5</term><term>Oligonucleotide</term><term>Oligonucleotide microarray analysis</term><term>Organic anion</term><term>Organic anion transporter</term><term>Organic cation transporter</term><term>Osmolyte</term><term>Osmolyte transporters</term><term>Parenchymal</term><term>Partial hepatectomy</term><term>Perfused</term><term>Physiol</term><term>Polypeptide</term><term>Protein kinase</term><term>Receptor</term><term>Regeneration</term><term>Ribosomal protein</term><term>Sham</term><term>Sham operation</term><term>Smit</term><term>Solute carrier family</term><term>Statistical analysis</term><term>Strong induction</term><term>Tissue inhibitor</term><term>Transport activities</term><term>Transporter</term><term>Transporter regulation</term><term>Uorescence</term><term>Uorescence signal</term><term>Upregulation</term><term>Uptake</term><term>Wettstein</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Aims: The aim of this study was to investigate the regulation of hepatic transport systems during liver regeneration. Methods: A DNA oligonucleotide microarray was developed with probes for 400 transcripts. Data were confirmed using real‐time PCR and on a functional level in the perfused rat liver. Liver homogenates were taken 3–48 h following 2/3‐hepatectomy in rats and compared with sham‐operated and non‐operated controls. Results: A more than two‐fold increase or decrease of expression was obtained in 183 genes following partial hepatectomy and in 16 genes in sham‐operated rats. A strong induction during liver regeneration was detected for the amino acid transporters LAT4, SN2 and sodium‐dependent neutral amino acid transporter (ASCT)2, whereas amino acid transport system (ATA)2 and ATA3 expressions remained unchanged. The upregulation of ASCT2 may be responsible for the increase in sodium‐dependent neutral amino acid influx important for liver cell proliferation. Expression of the osmolyte transporters Smit, TauT and Bgt1 was almost unchanged indicating that osmolytes are not involved in the cell volume increase during liver regeneration. The basolateral bile salt transporter Ntcp messenger RNA (mRNA) was significantly downregulated, whereas bile salt export pump (Bsep) and multidrug resistance protein (Mrp)2 expressions remained almost unchanged. An increased mRNA expression following partial hepatectomy was detected for organic anion transporting polypeptide (Oatp)5, Octn1, Octn2 and SGLT2. In contrast, Mrp6, Oatp 2, Oatp 3, Oatp 4 and Oatp 7 were downregulated. A five‐fold upregulation at the protein level was shown for the Na+–K+–2Cl− cotransporter sodium‐potassium‐2‐chloride cotransporter (NKCC1). Conclusions: The data show a differential regulation of hepatic transport systems during liver regeneration.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country></list><tree><country name="Allemagne"><noRegion><name sortKey="Dransfeld, Olaf" sort="Dransfeld, Olaf" uniqKey="Dransfeld O" first="Olaf" last="Dransfeld">Olaf Dransfeld</name></noRegion><name sortKey="Gehrmann, Thor" sort="Gehrmann, Thor" uniqKey="Gehrmann T" first="Thor" last="Gehrmann">Thor Gehrmann</name><name sortKey="H Ussinger, Dieter" sort="H Ussinger, Dieter" uniqKey="H Ussinger D" first="Dieter" last="H Ussinger">Dieter H Ussinger</name><name sortKey="Holneicher, Claudia" sort="Holneicher, Claudia" uniqKey="Holneicher C" first="Claudia" last="Holneicher">Claudia Holneicher</name><name sortKey="Kircheis, Gerald" sort="Kircheis, Gerald" uniqKey="Kircheis G" first="Gerald" last="Kircheis">Gerald Kircheis</name><name sortKey="Kohrer, Karl" sort="Kohrer, Karl" uniqKey="Kohrer K" first="Karl" last="Köhrer">Karl Köhrer</name><name sortKey="Wettstein, Matthias" sort="Wettstein, Matthias" uniqKey="Wettstein M" first="Matthias" last="Wettstein">Matthias Wettstein</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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